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Figure 1(a). A culture of live Madin-Darby Canine Kidney cells stained with DiOC6(3) at a high concentration observed using phase optics. Cells were treated with 500 nM DiOC6(3), staining the mitochondria and endoplasmic reticulum of the live MDCK cells. Cells were observed using phase optics. The nucleus (N), including the nuclear envelope and the nucleoli, is clearly visible, in addition to many lipid droplets (LD) within the cell. The mitochondria (M) are difficult to distinguish from the cell body using phase optics, and the endoplasmic reticulum (ER) is virtually indistinguishable.

Figure 1(b). A culture of live Madin-Darby Canine Kidney cells stained with DiOC6(3) at a high concentration observed using fluorescence optics. The same cells as in Figure 1(a) were observed using fluorescence optics. The nucleus (N) can be located due to the absence of mitochondria (M) or endoplasmic reticulum (ER), but details of the nucleus such as the nuclear envelope or nucleoli are unseen. Lipid droplets (LD) are not visible under fluorescence microscopy because they are not tagged with DiOC6(3). The mitochondria are easily visible throughout the cell, and the endoplasmic reticulum is visible as a lattice-like structure.

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2 years ago, 6 notes
#science #bio #mount holyoke #cell bio #microscopy #fluorescence #mdck cells #madin-darby canine kidney cells #phase optics #fluorescence optics #microscope #micro #school #college #lab #live #mine #neuro #cells #optics #lipid droplets #mitochondria #endoplasmic reticulum #nuclear envelope #nucleoli #nucleus

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